Production of histological preparations for the treatment of clinical and cell biological issues
The unit represents a technical platform of the German Diabetes-Center and is associated to the research programs A and B. For investigation of clinical and cell biology questions, histological samples are generated (human biopsies, mouse tissues), processed, and subsequently analyzed by light- and electron microscopy, respectively. The establishment and further improvement of new methods, such as ultrastructural analytical procedures with immunogold electron microscopy and quantitative morphometric analyses, expands the experimental options of the institute and may allow further integration of the research programs at the DDZ.
Team
Selected Publications
- Gancheva S, Ouni M, Jelenik T, Koliaki C, Szendroedi J, Toledo FGS, Markgraf DF, Pesta DH, Mastrototaro L, De Filippo E, Herder C, Jähnert M, Weiss J, Strassburger K, Schlensak M, Schürmann A, Roden M 2019. Dynamic changes of muscle insulin sensitivity after metabolic surgery. Nat Commun. 10. https://doi.org/10.1038/s41467-019-12081-0
- Wardelmann K, Blümel S, Rath M, Alfine E, Chudoba C, Schell M, Cai W, Hauffe R, Warnke K, Flore T, Ritter K, Weiß J, Kahn CR, Kleinridders A 2019. Insulin action in the brain regulates mitochondrial stress responses and reduces diet-induced weight gain. Mol Metab. 21: 68-81. https://doi.org/10.1016/j.molmet.2019.01.001
- Stermann T, Menzel F, Weidlich C, Jeruschke K, Weiß J, Altenhofen D, Benninghoff T, Pujol A, Bosch F, Rustenbeck I, Ouwens M, Thoresen GH, de Wendt C, Lebek S, Schallschmidt T, Kragl M, Lammert E, Chadt AS, Al-Hasani H 2018. Deletion of the RabGAP TBC1D1 leads to enhanced insulin secretion and fatty acid oxidation in islets from male mice. Endocrinology. 159: 1748-1761. https://doi.org/10.1210/en.2018-00087
- Kragl M, Schubert R, Haiko K, Otter S, Bartosinska B, Jeruschke K, Weiß J, Chen C, Alsteens D, Kuss O, Speier S, Eberhard D, Müller DJ, Lammert E 2016. The biomechanical properties of an epithelial tissue determine the location of its vasculature. Nat Commun. 7. https://doi.org/10.1038/ncomms13560
- Lambernd S, Taube A, Schober A, Platzbecker B, Görgens SW, Schlich R, Jeruschke K, Weiss J, Eckardt K, Eckel J 2012. Contractile activity of human skeletal muscle cells prevents insulin resistance by inhibiting pro-inflammatory signalling pathways. Diabetologia. 55: 1128-39. https://doi.org/10.1007/s00125-012-2454-z
Cooperations
- Deutsches Institut für Ernährungsforschung, Nuthetal: A. Kleinridders
- Heinrich-Heine-Universität Düsseldorf: A.Gödecke
Teaching activities at Heinrich Heine University Düsseldorf
- Scientific work instructions / Cell Morphology (Weiß)